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After 10 min at 30C, H 2 O 2 was removed with two centrifugation steps at 1,500 g for 3 min at room temperature and following resuspension in isosmotic buffer to a final concentration of 1.5 OD 600 units/ml

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A simple approach to ensuring adequate rotation is the clock method: imagine each injection region as a clock face and inject at a different hour position each time you use that region

The compound buthionine sulfoximine (BSO, Figure 2D) can induce ferroptosis in retinal pigment epithelium by inhibiting glutamate-cysteine ligase (GCL), a rate-limiting enzyme in de novo GSH synthesis (Sun et al., 2018)

Wang, E.E